Factor VIII Assay 161

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Factor VIII Assay - 161

Name	Method	Reference Range	Units
Factor VIII Activity	CLOT DET	50 - 150	%

No	Type	Volume	Temperature	Container Type	Instructions
1	Plasma Citrated - Primary	2 (1) mL	Frozen - 14 Day(s)	Light Blue	See COLLECTION INSTRUCTIONS for platelet-poor plasma.

Setup Schedule	Monday, Thursday, Saturday
Reported	2 days
CPT Code(s)	85240
Notes
Performing Lab	Specialty Laboratories
Clinical Utilities	Low Factor VIII activity can be an indication of Hemophilia A or von Willebrand disease. Acquired FVIII deficiency is seen in 15% of severe Hemophilia A patients treated with FVIII concentrates. Acquired deficiency is also noted in older individuals without underlying disease, as well as in post partum women. Elevated FVIII activity is a risk factor for thrombosis.


Instructions for platelet-poor plasma: 1. Draw a plain red top tube to remove tissue fluid contamination. Discard this tube. 2. Draw blood into a buffered citrate collection tube (light blue top) filled to proper level. Do not overfill. 3. The blood-to-anticoagulant ratio should be 9:1; inadequate filling of the collection device will decrease this ratio and may lead to inaccurate results. 4. Adjust the final citrate concentration in the blood of patients who have hematocrit values above 0.55 (55%). For hematocrits below 20%, there is no current data to support a recommendation for adjusting the citrate concentration. 5. The needle gauge could be between 19-22. For pediatric patients a 21-23 gauge needle may be used. 6. Invert gently 6 times to mix. Process immediately. 7. Centrifuge for 15 min at 2500 x g. 8. Remove plasma using a plastic pipette to transfer into a new tube. 9. Repeat centrifugation at 2500 x g for 15 minutes to assure complete platelet removal. 10. Dispense the plasma into 2 or more plastic tubes using a plastic transfer pipette. Label tubes appropriately. 11. Freeze immediately at -70 C. 12. Specimen must remain frozen at all times. Ship to Specialty within 24 hours on dry ice. 13. Specimen should not be submitted if: * it is hemolyzed * microclots are present * the tube is less than 90% filled * a specimen with hematocrit >55% is collected without anticoagulant adjustment.

Instructions for platelet-poor plasma:
1. Draw a plain red top tube to remove tissue fluid contamination.
Discard this tube.
2. Draw blood into a buffered citrate collection tube (light blue
top) filled to proper level. Do not overfill.
3. The blood-to-anticoagulant ratio should be 9:1; inadequate
filling of the collection device will decrease this ratio and
may lead to inaccurate results.
4. Adjust the final citrate concentration in the blood of
patients who have hematocrit values above 0.55 (55%). For
hematocrits below 20%, there is no current data to
support a recommendation for adjusting the citrate concentration.
5. The needle gauge could be between 19-22. For pediatric
patients a 21-23 gauge needle may be used.
6. Invert gently 6 times to mix. Process immediately.
7. Centrifuge for 15 min at 2500 x g.
8. Remove plasma using a plastic pipette to transfer into a
new tube.
9. Repeat centrifugation at 2500 x g for 15 minutes to assure
complete platelet removal.
10. Dispense the plasma into 2 or more plastic tubes using a
plastic transfer pipette. Label tubes appropriately.
11. Freeze immediately at -70 C.
12. Specimen must remain frozen at all times. Ship to Specialty
within 24 hours on dry ice.
13. Specimen should not be submitted if:
* it is hemolyzed
* microclots are present
* the tube is less than 90% filled
* a specimen with hematocrit >55% is collected without anticoagulant
adjustment.

PLEASE NOTE: CPT codes are to provide guidance. These codes are subject to change on a regular basis and it is the responsibility of the client to verify accuracy of the codes listed. You should refer to the most current version of the CPT coding manual published by the American Medical Association to resolve any issues.

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